1
Insert the lipoaspirate into the separator through any of the channels labeled 1 to 4. The total volume of the lipoaspirate should be between 50 and 200 ml.
To control the insertion volume, use the scale on the flask.

2

Insert the lipoaspirate into the separator through any of the channels labeled 1 to 4. The total volume of the lipoaspirate should be between 50 and 200 ml.
To control the insertion volume, use the scale on the flask.

3
Place the separator in a shaker incubator, set the stirring speed to 100-120 rpm and a temperature of 37 ° C. Stir for 30 minutes.

4
Centrifuge the separator in a centrifuge at 300 g for 10 min.

5
Consistently, starting from the shorter channel (marking 1), select the supernatant (namely, split adipose tissue, oil and wash solution) through channels labeled 1 to 4 and Clean (marked with a green ring). The fluid should remain only in the lower chamber of the separator.

6
With a Hartman solution, bring the volume of liquid in the separator to 400 ml. Centrifuge the separator in a centrifuge at 300 g for 10 min.

7
Carefully remove the entire volume of liquid from the upper chamber of the separator with a sterile syringe through the channel marked Clean (marked with a green ring). Carefully select the entire volume of fluid (stromal-vascular fraction) from the lower chamber of the separator through a channel marked SVF (indicated by a pink to red color ring) with a sterile syringe.

EQUIPMENTNecessary equipment

Centrifuge

Centrifuge

Working with the ESVIEF System use properly registered centrifuges with the appropriate volume and size of buckets.

Shaker incubator

Shaker incubator

Working with ESVIEF System use properly registered shaker incubators

Hartman’s solution

Hartman’s solution

Buffer solution for washing adipose tissue and SVF

Syringes with Luer Lock connector

Syringes with Luer Lock connector

A syringe such as Luer Lock is also called a “twist”, as it has a screw connector.